SYBR Green I
coady at ERE.UMontreal.CA
Tue Sep 6 19:47:44 EST 1994
In article <Cvq854.2wr at ncifcrf.gov> pnh at fcsparc6.ncifcrf.gov (Paul N Hengen) writes:
>I don't think the DNA is diffusing, but the EtBr is coming off the DNA and
>then the bands look fuzzy under UV. I think this because you can re-stain your
>gel with EtBr and see good bands again. If you do the same thing with SYBR
>Green I, there is very little diffusion from the DNA and your bands still look
>"tight" after several days in buffer. I've noticed though that SYBR Green I
>degrades very quickly when left out at room temperature and goes off within a
>few days if not stored in a brown bottle. This makes me think that some
>wavelength of visible light can be used to rid the fluorescence (and maybe the
>DNA binding capacity?) of the dye. It would be nice if this wavelength were
>found not to be harmful to the DNA so that the stain could just be removed by
>exposure to some specific light source.
I guess sunlight is destroying the fluorescent dye, but
this should not necessarily be construed as removing the stain from
the DNA. It may well be there but simply no longer be fluorescent,
due to some photochemical change.
Michael J. Coady
COADY at ERE.UMONTREAL.CA
The opinions expressed above are solely those of the author and do not,
in any way, shape or form, represent the Universite de Montreal.
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