gel pull off

Dmitry Zharkov dmitry at pharm.som.sunysb.edu
Tue Sep 6 17:22:33 EST 1994


Hi All,
I recently had an interesting trouble with 12% PAGE. When I started pre-run at
1500 V, 50 mA, after about 10 min the gel was pulled off from the top of the plates
into the upper reservoir. When I repeated and pre-ran gel at 500 V, this did not
occur; however, when I increased a voltage, it was pulled again. I never
experienced it with 20% gels (with 8M urea, in both cases). What might be a
cause?
Thanks for explanations!
D.Zharkov (dmitry at pharm.som.sunysb.edu)



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