raising ANTIBODIES with fusion proteins??
fodde at ruly46
Tue Sep 6 17:49:12 EST 1994
We have made several his-tagged fusionproteins which we used to immunize
rabbits. The sera (and also affinity-purified antibody) of these rabbits
recognize on western-blots besides the expected band, also extra bands. Does
anyone know a method to get rid of these antibodies recognizing the wrong
bands? We are thinking of making subfragments of the original fusionprotein
and then affinity purify the sera, hoping the aspecific antibodies don't
recognize the subfragment but there must be more clever ways to do this?
Besides this request for help I wonder what the current explanation is for the
generation of these aspecific antibodies. All reactions and suggestions are
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