transfection of monocytic cells

Markus Guetlich guetlich at haema103.gsf.de
Wed Sep 7 06:30:58 EST 1994


We tried to transfect the monocytic cell lines U937 and  THP-1 by
electroporation with the electroporator bio rad gene pulser. The U937 could
not be transfected by any variation of capacity and voltage. Perhaps anyone
has more succeed in this, it would be nice to tell me. The transfection of
THP-1 worked bad under our standard conditions for transfecting the raji
B-cells (250 V,960uF). For any advice I thank you previously.

With best wishes

Klaus Witter 



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