Seq Gel Spots"
mitcheld at rocbi.DNET.roche.com
Wed Sep 7 09:33:19 EST 1994
Robert Minto writes
>I am in need of some help removing interfering black dots from my
>polyacrylamide sequencing gels. The gels are prepared by the U.S.
>Biochemical Sequencing support guide protocol which contains 8M urea (and
>appears to be identical to Maniatis, 6% acrylamide, 19:1
>acrylamide:bisacrylamide). The gels contain black dots which are
>concentrated at the top of the sequencing gel and are ONLY in the lanes.
>They actually interupt the lanes, suggesting particles in the gel. (Stuff
Saw exactly the same problem a couple of years ago. Turns out is was a problem
with the stop buffer from the sequencing kit I was using. When I threw that
particular tube away and used one from a different kit the problem disappeared.
This explained why it only appeared in the wells and why (as a later poster
points out) they are only in the top third of the gel.
Mitcheld at rocbi.dnet.roche.com
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