PCR: Promega Taq vs. Perkin-Elmer Taq
zainul at KB.USM.MY
Thu Sep 8 11:04:27 EST 1994
We have evaluated Taq from a number of sources including Promega and
Perkin-Elmer. We found that Promega Taq is okay with purified(ish) DNA
but are not too reliable when it comes to using clinical specimens. Taq
from PE and Boeringer-Mannheim (also licensed) are far superior for
amplifying targets in clinical specimens.
Zainul F. Zainuddin
On 6 Sep 1994, Ross Whetten wrote:
> In article <346gh9$dhs at neuro.usc.edu>, merlin at neuro.usc.edu (merlin) writes:
> |>Promega Taq seems significantly cheaper Perlin-Elmer Taq. Are there
> |>any significant differences between these two enzyme sources for PCR?
> Promega Taq is not licensed for use in PCR;
> the difference in cost between Promega enzyme and
> Taq from BMB, USB, Perkin-Elmer or other sources is
> due in part to the royalties paid by the other vendors
> to Hoffman-LaRoche.
> Ross Whetten
> Research Assistant Professor
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