PCR from intact bacterial cells

Seth Daniel Crosby dd034 at cleveland.Freenet.Edu
Thu Sep 8 09:37:46 EST 1994


Yes, the trick is to just barely touch the bacterial colony
with your yellow tip befor dipping it into the PCR mix.  If you
can SEE a glob on the yellow tip, you already have too much.
20-25 cylces ought to do it.
Seth Crosby



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