RNA tips

Chutney devitta at uk.ac.birmingham
Fri Sep 9 09:28:14 EST 1994


In article <Stephen_Lasky-0909940903120001 at tonto-slip12.cis.brown.edu>,
Stephen_Lasky at brown.edu (Stephen R. Lasky) wrote:

> In article <34o1tl$1un at gazette.bcm.tmc.edu>, ah690549 at mbcr.bcm.tmc.edu
> (Annette C. Hollmann) wrote:
> 
> > In article <34nqf2$dkt at cdc1.cdc.gov> parsons at METEOR.BIOTECH.CDC.GOV writes:
> > >Hello,
> > >       I am looking for some tips on working with RNA.  Specifically,
I would
> > >like to know how people treat their plasticware. 
> <<<<Delete>>>>>
> > 
> > If all your solutions are made with DEPC-treated ddH2O, then the only
> > remaining culprit would be the gel unit.
> > If it is made of chloroform resistant material you can just wipe it with a
> > chloroform-soaked kimwipe and let it air-dry (USE THE FUME HOOD FOR THIS)
> > If it cannot be cleaned with chloroform, you have to treat it with dilute
> > DEPC (described in Maniatis et al)
> > Annette
> > ah690549 at mbcr.bcm.tmc.edu
> 
> We generally treat our gel boxes and combs with 0.1 N NaOH overnight and
> then wash them to neutrality with DI water (Most of the times this is DEPC
> treated water but as pointed out by another resopnder, if your water
> system is in good shape this is probably not necessary.)
> 
> As far as plastic goes, it is safe to assume that virgin plastic is free
> of RNases
> 

3% hydrogen peroxide is the most efficient way of cleaning plastic.  Soak
in H2O2 for 1 hour to overnight.  Rinse with DEPC-treated water and let
dry.
Generally though tips etc need not be cleaned, just autoclaved assuming
that you have only touched the items with gloves on
Andrew Devitt

> I presume that your fomaldehyde and formamide are deionized.  If not, you
> might try doing that.
> 
> Hope that helps
> 
> SRLasky
> 
> ***************************************************************
> Stephen R. Lasky, Ph.D.       Brown University/Roger Williams Medical Center
> e-mail: Stephen_Lasky at brown.edu         LandLine: 401-456-6572
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> A man said to the Universe: "Sir, I exist!"
> "However," replied the Universe, "the fact has not created in me a sense
of obligation."
>       -- Stephen Crane
> ***************************************************************



More information about the Methods mailing list