Precip. DNA from agarose "squeeze" ?

the End jgraham at bronze.ucs.indiana.edu
Sun Sep 11 19:40:52 EST 1994


Although I usually use DNA removed from agarose gels directly in the 
"eluate" (eg. freeze-squeeze, filtered, ect), this time I need 
to precipitate first.

In my hands, I never get good precipitation when DNA is in the 
presence of residual agarose left after the simple and effective
extraction procedures. I usually use 0.5 vol of ammonium acetate 
as counter ion and 3 vol of ethanol.

Anyone got a way ?

Thanks,

Please E-mail me a direct copy this time.

Jim
J. E. Graham 
Biology and Chemistry Departments 
Indiana University Bloomington 




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