Precip. DNA from agarose "squeeze" ?
jgraham at bronze.ucs.indiana.edu
Sun Sep 11 19:40:52 EST 1994
Although I usually use DNA removed from agarose gels directly in the
"eluate" (eg. freeze-squeeze, filtered, ect), this time I need
to precipitate first.
In my hands, I never get good precipitation when DNA is in the
presence of residual agarose left after the simple and effective
extraction procedures. I usually use 0.5 vol of ammonium acetate
as counter ion and 3 vol of ethanol.
Anyone got a way ?
Please E-mail me a direct copy this time.
J. E. Graham
Biology and Chemistry Departments
Indiana University Bloomington
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