Removal of Dna Probes

Emma Macfarlane emma at HERA.MED.UTORONTO.CA
Mon Sep 12 09:02:05 EST 1994

With Amersham's Hybond N+ membranes and fluroscein labelled DNA probes
I have had no problems removing the probe as follows. Bring a solution
of 0.1% (w/v) SDS to the boil, add membrane and allow solution to cool
to room temp. I don't see any reason why this also shouldn't work 
with 32P-labelled probes and other membranes.
Hope this is some help
P.S. If your blot has been allowed to dry out at any stage this also
prevents removal of the probe.

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