Removal of Dna Probes

Emma Macfarlane emma at HERA.MED.UTORONTO.CA
Mon Sep 12 09:02:05 EST 1994


With Amersham's Hybond N+ membranes and fluroscein labelled DNA probes
I have had no problems removing the probe as follows. Bring a solution
of 0.1% (w/v) SDS to the boil, add membrane and allow solution to cool
to room temp. I don't see any reason why this also shouldn't work 
with 32P-labelled probes and other membranes.
Hope this is some help
Emma
P.S. If your blot has been allowed to dry out at any stage this also
prevents removal of the probe.



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