darreng at ccmar.csiro.au
Mon Sep 12 23:14:51 EST 1994
In article <sticknbd-110994224146 at 220.127.116.11> sticknbd at miranda.cc.vanderbilt.edu (B Stickney) writes:
>In addition to the recommendations of others, with which I agree, I
>K treat my gel boxes if I have used them for anything besides an RNA gel
>the last one.
> > JoeJoe:
> > DEPC: I never use it. Besides, it's a carcinogen, and it can modify
>Nick, I've heard this before. How does DEPC modify RNA? No one I know
>seems to have any details about this.
According to Maniatis "DEPC modifies purine residues in RNA by
carboxymethylation" This apparently reduces RNA translation but does not
seriously alter the molecule's ability to form RNA:RNA or RNA:DNA hybrids.
CSIRO. Dept. Animal Production.
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