RNA tips

Darren Greeve darreng at ccmar.csiro.au
Mon Sep 12 23:14:51 EST 1994


In article <sticknbd-110994224146 at 160.129.125.10> sticknbd at miranda.cc.vanderbilt.edu (B Stickney) writes:
<stuff deleted>

>JoeJoe:
>In addition to the recommendations of others, with which I agree, I
>proteinase
>K treat my gel boxes if I have used them for anything besides an RNA gel
>since
>the last one.

> > JoeJoe:
> > DEPC: I never use it.  Besides, it's a carcinogen, and it can modify
>RNA.

>Nick, I've heard this before.  How does DEPC modify RNA?  No one I know
>seems to have any details about this.
>B Stickney

According to Maniatis "DEPC modifies purine residues in RNA by 
carboxymethylation" This apparently reduces RNA translation but does not 
seriously alter the molecule's ability to form RNA:RNA or RNA:DNA hybrids.

D.Greeve
CSIRO. Dept. Animal Production.
Western Australia.



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