columns for miniprep dna

eb86336 at inet.d48.lilly.com eb86336 at inet.d48.lilly.com
Mon Sep 12 18:28:40 EST 1994


We had problems sequencing Wizard miniprep DNA as well.  Our solution was to
combine 3 Wizard preps (150 ul), add 350 ul dH2O, Microcon-30 spin at 13,800 xg
for 10 min., QS back to 150 ul with dH2O and sequence.  We were seeing a salt
problem with the Wizard preps that did not occur with the Magic preps.  This
technique has not failed us yet and we sequence almost everything.

Mike Menke
Eli Lilly and Company



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