help with protein expression

eb86336 at inet.d48.lilly.com eb86336 at inet.d48.lilly.com
Mon Sep 12 17:59:59 EST 1994


What promoter system are you using?  What organism is the gene from?  What is
the GC content of your gene?  How good is the genes ribosome binding site
(Shine-Dalgarno)?  Are you using an expression system that utilizes the genes
SD sequence or do you have a system that you only clone in ORF cassette?  Could
be mRNA secondary structure maksing the SD sequence.  

If you give me some more info., I might be able to help more with this problem.

Mike Menke
Eli Lilly & Company



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