artifact bands on sequencing gels

L. Fred Jerva jerva at ELIRIS.MED.YALE.EDU
Mon Sep 12 18:16:17 EST 1994


I have artefacts at one particular locus of a cDNA sequence where
the actual sequence is GGGTT.  The sequencing gel shows bands of
equal intensity in both the G and T lanes where only Gs should be
present.  This is a double-strand sequencing reaction with Sequenase
2.0.  Does anybody have any suggestions on how to remove this 
artefact?

Fred




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