info about suicide vectors systemsIs2
PANIAGUA SOLIS JORGE FERNANDO
jpaniagf at CONDOR.DGSCA.UNAM.MX
Mon Sep 12 16:09:26 EST 1994
This is the first time that I dare to participate in this panel.
I am trying to substitute a wild/type gene for a defective one in
Salmonella. I am clonig the defective construct in a pir based suicide
plasmid in the SY327lambda pir E. coli strain. I have received such
system from someone else in the lab and they dont have more information
about it. I have 3 questions:
a) Does anyone out there knows the genotype of the SY327 lambda pir?
b) Is there a particular transformation procedure for such strain?
c) Is there an alternative to the use of suicide vectors or phage
transduction to knock/out genes in Salmonella?
jpaniagf at condor.dgsca.unam.mx
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