glycoprotein purification

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Sep 12 15:01:49 EST 1994


In Article <34nj0i$bmo at thot.u-strasbg.fr>, nussbaum at neurochem.u-strasbg.fr
wrote:
>I have an antiserum which was obtained by injecting a rabbit with an
> N-terminal sequence of an until now undescribed protein. this antiserum 
>reacts specifically with a protein present in a wheat germ agglutinin 
>fraction isolated from rat brain. I am loking for ideas or suggestions to go
> further in the purification of this antigen by another way than that I have
>used (Western blotting). Note that this protein is solubilized by DOC and 
>probably other detergents. Purification of this antigen by immunoaffinity
column
> chromatography is, in my opinion, not feasible since the antibodies are not
> strong enough. I am thinking about ion exchange chromatography or similar
>things. thanks in advance for your help.
>Nussbaum
>E-mail: nussbaum at neurochem.u-strasbg.fr

You might try Triton X-114 to selectively solubilize glycoproteins.

Tracy Aquilla, Ph.D.
Molecular Physiology and Biophysics
University of Vermont
aquilla at salus.med.uvm.edu



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