T-tailing for PCR product ligation

Paul A. Vescio vescip at rpi.edu
Mon Sep 12 06:40:00 EST 1994


Current Protocols In Molecular Biology has a procedure for this that works
fairly well if your PCR products aren't to old.

In Article <350q8o$24d at styx.uwa.edu.au>, lsellner at uniwa.uwa.edu.au (Loryn
Sellner) wrote:
>
>Dear netters,
>I have been reading the advice for cloning of PCR products by using the 
>TA cloning vector (or any T tailed vector). Is there a way to T tail my 
>own vector so I can clone my PCR product into the site I want, (namely a 
>blunt ended restriction site)?
>Thanks.
>Loryn
>
>
--------------------------------------------------------------------------------
Paul A. Vescio                      
Biology Dept.
Rensselaer Polytechnic Institute

!!New York Rangers  Stanley Cup Champions 1994!! 



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