In Vivo Excision of ZapII

Jon Burrows jb at scs.unr.edu
Tue Sep 13 18:32:43 EST 1994


	I am attempting to excise the entire library from a lambda ZapII library
into pBluescript form. I intend to use this plasmid DNA in the screening 
protocol recently described by Pippin et al. Biotechniques, 16, 676, 1994.
	I am interested in any advice concerning the subsequent
conversion of phagemid ssDNA to plasmid dsDNA. In particular how to achieve
"200 micrograms" of purified plasmid from the library containing a full 
representation of the cDNAs.
	Thanks in advance for your comments.
	Jon Burrows.



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