PCR directly from phage

Harry Menegay hxm8 at po.cwru.edu
Tue Sep 13 15:34:30 EST 1994


I'm trying to PCR out of a phage cDNA library to determine
if my gene of interest is located in the library before I
go ahead and screen the library.  My question is how many 
phage can you put in a PCR reaction before the reaction dies
due to too much protein from all the phage?  Anyone tried this?

Thanks for any help
Harry



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