ABI sequencing artifact

Matthew Stoecker quinnt at u.washington.edu
Wed Sep 14 22:02:42 EST 1994


I have been using the ABI DyeDeoxy kit with Taq for sequencing and have
encountered a strange artifact.  On one particular template, I get
beautiful read for about 200 bp, then spotty read for about 10 bp, then
absolutely nothing for the rest of the run.  This has happened twice, and
the break always occurs in the same spot.  I was wondering if anyone had
any similar occurences or any ideas about what's going on.  Thanks a
lot!!!

Matthew Stoecker
U. of Washington



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