Tightly regulated E. coli Promoters

ez001427 at dale.ucdavis.edu ez001427 at dale.ucdavis.edu
Wed Sep 14 19:59:57 EST 1994


Dear Philip-

	Have you tried using the T7 RNA polymerase promoter, and inducing 
by addition of phage lambda CE6?  In E. coli strains without the T7 RNA 
polymerase, you will get no expression of your target gene, because of 
dissimilarities between E. coli promoters and the T7 promoter.  Toss CE6 
phage into the culture, they crank out the t7 RNA polymerase, and your 
gene turns way on.  One possible drawback for you is that the cells will, 
uh, die.  But that will take a while, and you may be able to conduct your 
experiment in the interim.

Marc Goldstein
magoldstein at ucdavis.edu




More information about the Methods mailing list