Electroporation Improvements

Duncan Clark Duncan at genesys.demon.co.uk
Wed Sep 14 07:44:28 EST 1994


In article: <34r2e0$2dp at lace.Colorado.EDU>  srutt at beagle.Colorado.EDU (Ben-Sapiens) writes:


> live cells per 50ul sample = 7.5X10^7 (30X lower than literature)
> cell survival after electroporation = near 100%
> fraction of surviving cells which receive plasmid = 67%

What happens if you concentrate the cells more. You should be taking 1 
litre down by at least 500 fold ie 2ml. At your 1.5OD you should have 
roughly 10e8 cells/ml so concn to 2ml should give you 2.5x10e9 in 
50ul. How come you only have 7.5x10e7? 



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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
G4ELJ                               | Compuserve:  100015.1406 at compuserve.com
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