Diff. display problems

HARDIES at THORIN.UTHSCSA.EDU HARDIES at THORIN.UTHSCSA.EDU
Thu Sep 15 14:46:59 EST 1994


G. Lanre Babalola (Babalola at mail.sas.upenn.edu) wrote:

> I do the differential display RT/PCR routinely.  ...
> [Some lanes fail in the sense that they] had intense radioactive signals
> hanging at the wells while the "good reactions" never had this amount at
> the wells.  ...

If you interposed an EtOH or other precipitation between the PCR rxn
and the gel, failure to completely redissolve the aggregated DNA
might stick it in the slot.  ???

Steve Hardies, Dept. of Biochem., Univ. of Texas HSC at San Antonio
Hardies at thorin.uthscsa.edu




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