RNA tips (DEPC and nucleic acid)

madani at vms.cis.pitt.edu madani at vms.cis.pitt.edu
Thu Sep 15 10:56:12 EST 1994


Hi, correct me if I am mistaken, but I was always under the 
impression, that DEPC inhibits nucleases by modifying His and Tyr
residues. Also, that DEPC slowly hydrolyzes to yield carbon-
dioxide and ethanol, a process which can be effectively
accelerated by autoclaving DEPC-treated water for 45 minutes or so.

Navid.



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