Method for Touchdown PCR

LOGAND logand at msdos.montpellier.inra.fr
Fri Sep 16 04:03:48 EST 1994


Dear All,

I wish to determine the specificity of a PCR reaction with regard to the 
anealing temperature and "think" that Touchdown PCR is an efficient means to 
this end. However, I do not entirely understand the method (having only seen it 
mentioned in brief on the net) and would thus be very grateful to anyone who 
could enlighten me via the net or by faxing the method of Roux, K.H. In 
Biotechniques 16(5) : 812. We do not get Biotechniques here. 

Thanks in advance


David C. Logan
INRA-ENSA(M)-CNRS
Montpellier
FRance

Logand at inra.montpellier.fr

fax: (int. code) 67-52-57-37




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