Method for Touchdown PCR

Wiggy A.C.Hilton at bham.ac.uk
Fri Sep 16 06:37:44 EST 1994


In article <35bn1k$cif at mserv1.dl.ac.uk>, LOGAND
<logand at msdos.montpellier.inra.fr> wrote:

> Dear All,
> 
> I wish to determine the specificity of a PCR reaction with regard to the 
> anealing temperature and "think" that Touchdown PCR is an efficient means to 
> this end. However, I do not entirely understand the method (having only
seen it 
> mentioned in brief on the net) and would thus be very grateful to anyone who 
> could enlighten me via the net or by faxing the method of Roux, K.H. In 
> Biotechniques 16(5) : 812. We do not get Biotechniques here. 
> 
> Thanks in advance
> 
> 
> David C. Logan
> INRA-ENSA(M)-CNRS
> Montpellier
> FRance
> 
> Logand at inra.montpellier.fr
> 
> fax: (int. code) 67-52-57-37

Via the net would be good if possible as I too would like to know more and
am unable to get Biotechniques.

Wig.

-- 
      **********************************************************
      ** It's because I come from Wigan that my name is Wiggy,**
      ** and nothing to do with my head................Honest!**
      **                                                      **
      **                 A.C.Hilton at bham.ac.uk                **
      **********************************************************
                         



More information about the Methods mailing list