Method for Touchdown PCR

Wiggy A.C.Hilton at
Fri Sep 16 06:37:44 EST 1994

In article <35bn1k$cif at>, LOGAND
<logand at> wrote:

> Dear All,
> I wish to determine the specificity of a PCR reaction with regard to the 
> anealing temperature and "think" that Touchdown PCR is an efficient means to 
> this end. However, I do not entirely understand the method (having only
seen it 
> mentioned in brief on the net) and would thus be very grateful to anyone who 
> could enlighten me via the net or by faxing the method of Roux, K.H. In 
> Biotechniques 16(5) : 812. We do not get Biotechniques here. 
> Thanks in advance
> David C. Logan
> Montpellier
> FRance
> Logand at
> fax: (int. code) 67-52-57-37

Via the net would be good if possible as I too would like to know more and
am unable to get Biotechniques.


      ** It's because I come from Wigan that my name is Wiggy,**
      ** and nothing to do with my head................Honest!**
      **                                                      **
      **                 A.C.Hilton at                **

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