RACE Help me please!!

[Paiks]

In article <Cst7s0.HKv at ucdavis.edu>, ez015890 at chip.ucdavis.edu () writes:

I have tried 5'-RACE kit from BRL and got excellent results.
There's one catch though, I had to do three nesting of target specific
primers to get reproducible results. In addition, Boeringer-Manaheim Taq 
gave the best results.
Of course the RACE depends on the quality of total RNA to start with. I
use total RNA prepared with RNAzol.
Good luck.