protein overproduction in E. coli

Tracy Aquilla aquilla at
Fri Sep 16 13:50:52 EST 1994

In Article <940915092608.60201216 at>,
>I am teaching a biotechnology course in which I will talk about
>methods for overproduction of proteins in E. coli.  As examples
>of modern innovations I currently use the T7 system (as in 
>Novagen's pET system) to illustrate tight expression control, and
>the MalE fusion system to illustrate the use of a removable
>affinity purification handle.  Would anyone out there like
>to point me to other recent innovations that illustrate
>new principles?
>Thanks for your input.
>Steve Hardies, Dept. of Biochem., Univ. of Texas HSC at San Antonio
>Hardies at

I kind of like the baculovirus expression system, myself. This is another
twist that your students may find interesting.

Tracy Aquilla, Ph.D.
Molecular Physiology and Biophysics
University of Vermont
aquilla at

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