protein overproduction in E. coli

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Sep 16 13:50:52 EST 1994


In Article <940915092608.60201216 at thorin.uthscsa.edu>,
HARDIES at THORIN.UTHSCSA.EDU wrote:
>Hi,
>
>I am teaching a biotechnology course in which I will talk about
>methods for overproduction of proteins in E. coli.  As examples
>of modern innovations I currently use the T7 system (as in 
>Novagen's pET system) to illustrate tight expression control, and
>the MalE fusion system to illustrate the use of a removable
>affinity purification handle.  Would anyone out there like
>to point me to other recent innovations that illustrate
>new principles?
>Thanks for your input.
>
>Steve Hardies, Dept. of Biochem., Univ. of Texas HSC at San Antonio
>Hardies at thorin.uthscsa.edu
>

I kind of like the baculovirus expression system, myself. This is another
twist that your students may find interesting.

Tracy Aquilla, Ph.D.
Molecular Physiology and Biophysics
University of Vermont
aquilla at salus.med.uvm.edu



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