How to make random blunt end cuts in a plasmid?

BrianC4208 brianc4208 at aol.com
Fri Sep 16 09:55:01 EST 1994


I'm looking for a way to make random blunt end cuts in a plasmid.  I tried
DNAse, but I'm not totally convinced that DNAse makes true blunt cuts, and
I don't want to Klenow in the ends for fear of what I might do to my ORF. 
I appreciate any suggestions you might have.

Brian
BrianC4208 at aol.com



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