5' filling with Klenow

Jonathan M Kaplan jmk19 at namaste.cc.columbia.edu
Sat Sep 17 22:11:30 EST 1994


I have found that Klenow is reasonably reliable as long as you follow the
manufacturer's protocol.  Namely, only end fill for 15 minutes, this will
minimize any 3' --> 5' chewing.  

-Jon



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