Recommendations for light level protein detection

Dr. D.G. Fernig dgfernig at liverpool.ac.uk
Thu Sep 15 11:51:45 EST 1994


Hi,
A few suggestions:
The best methods for enhancing at the light level are
the PAP and the ABC kits.  Low background, high signal.
Methanol will kill off endogenous peroxidase very efficiently.
This brings up the method of fixation.  Methacarn (acidic
methanol fixative with 10% inhibisol) maintains mammalian
protein epitopes better than all the other fixatives we have
tried.  We then embed in paraffin etc.
Dave



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