CIP or BAP
bernard at elsie.nci.nih.gov
Sun Sep 18 12:34:44 EST 1994
In article <35ca22$9m3 at louie.udel.edu>, landel at helios (Carlisle Landel) writes:
> In article <35bo43$2k5 at aragorn.unibe.ch>, <beblum at ubeclu.unibe.ch> wrote:
> >RNA editing research group in Bern is
>requesting information about the use of CIP (calf intestinal alk. phosphatase)
>or BAP (bacterial alkaline phosphatase) in order to remove
>the 3' terminal phosphate from an RNA oligo. Which of the two works better for
>this special purpose?
> The enzyme of choice CIP, because it can be heat inactivated by incubation
> at 65 degrees, while BAP requires a phenol and chloroform extraction for
Another alternative is Shrimp Alkaline Phosphatase as this is very sensitive
to heat inactivation but is reportedly otherwise the same as CAP. I've used
SAP successfully but haven't performed a direct comparison. However, the
knowledge that the enzyme is efficiently killed is one less worry. I
don't have the deatils handy so I can't give you the supplier. I suspect
it is somewhat more expensive than CAP but one tube lasts for ages.
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)
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