RNA tips (DEPC and nucleic acid)

Eric C. Anderson anderson at pharmdec.wustl.edu
Mon Sep 19 10:33:33 EST 1994


In article <359qqu$lo2 at usenet.srv.cis.pitt.edu>, madani at vms.cis.pitt.edu
wrote:

> [snip] Also, that DEPC slowly hydrolyzes to yield carbon-
> dioxide and ethanol, a process which can be effectively
> accelerated by autoclaving DEPC-treated water for 45 minutes or so.

which is why when i make up DEPC solutions, i autoclave them first, _then_
add the DEPC once they cool down.

eric
-- 
*************************************
*Of course it's not true...         *
*But let's make the bastard deny it!*
*                        LBJ (1948) *
*************************************
eric c. anderson                                
anderson at pharmdec.wustl.edu
dept. of molecular bio. and pharm.               (314)362-3963 (lab)
washington univ. school of medicine              (314)862-2435 (home)
660 s. euclid box 8103                           (314)362-7058 (FAX)
st. louis, mo 63110



More information about the Methods mailing list