RNA Probe woes

D.Greeve <DAEMON> darreng at ccmar.csiro.au
Mon Sep 19 03:01:52 EST 1994


Hi netters.

   I am currently experiencing difficulties in producing P32 labeled RNA 
probes from a "Bluescript KS+"  plasmid. I am currently using a kit marketed 
by "Bresatec" (- an Australian company), designed to do this, but I am getting 
almost no transcription using T7 and my plasmid. The kit control appears to 
work, however with identical conditions, my plasmid does not.

Any ideas on the matter would be much appreciated....

Darren Greeve.



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