Liver RNA Prep Needed
darreng at ccmar.csiro.au
Mon Sep 19 02:53:46 EST 1994
In article <Cw8ox7.Ar6 at crash.cts.com> rcdana at crash.cts.com (Richard Dana) writes:
>Having trouble with avian liver RNA prep, need protocol. Brain, and
>heart and skeletal muscle preps are contaminated with DNA but have good
>yield. What can I do to minimize DNA contamination and obtain good liver
>RN for cDNA library construction.
Richard, I was having trouble with sheep liver/uterine/muscle RNA extractions.
I assume though you are looking at extracting mRNA, not total RNA as I was.
Try using the "Bresatec" RNA extraction reagents eg. Ultraspec or RNAzol B.
They seem to reduce yields slightly but clean up all DNA/Protein
contamination. From here I would guess that a CsCl prep to separate the mRNA
would be the way to go.
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