CIP or BAP

matthews matthews at oyster.arris.com
Mon Sep 19 11:58:12 EST 1994


I'm a firm believer in shrimp alkaline phosphatase (SAP). I normally
add 0.5U SAP per microgram of vector DNA directly to my restriction
digests, incubate for 1hr @ 37C and then heat to 68C for 30 minutes to
kill the SAP. At this stage I normally gel purify the vector before
ligation.

David Matthews

matthews at arris.com



More information about the Methods mailing list