Liver RNA Prep Needed

Ola Myklebost olam at radium.uio.no
Tue Sep 20 05:07:07 EST 1994


I use the good old Guanidine thiocyanate /CsCl prep, largely as described
in Maniatis. The pelleting of RNA through CsCl seems to remove DNA very
efficiently. I don't believe anything would work better (I have tried many
procedures). If you purify mRNA with oligo-dT magnetic beads (Dynal), very
little DNA should be carried through.

Ola

-- 
Ola Myklebost                   Email  ola.myklebost at labmed.uio.no
Dept of Tumor Biology
Inst for Cancer Research        Tel +47-2293-4299
The Norwegian Radium Hospital   Fax +47-2252-2421
N-0310 OSLO, Norway



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