Transients-DMSO shock.

George W Chacko gchacko at
Tue Sep 20 14:26:46 EST 1994

I was going through the Red Book transient transfection protocol by
the DEAE-dextran method and the recommendation made is to make a 10%
DMSO solution in 1x PBS. Is there any specific reason for this or
could 10% DMSO in DMEM be substituted?



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