RNA tips (DEPC and nucleic acid)

LKM100F at ODUVM.BITNET LKM100F at ODUVM.BITNET
Tue Sep 20 10:15:50 EST 1994


Michele Young wrote that the point of autoclaving solutions with DEPC was
to break it down so that one doesn't have to worry about working with a
potential carcinogen.
   Not only that, but the presence of DEPC can inhibit some enzymes such
as DNA and RNA polymerases.  So, you can defeat the purpose of your method
if you're not careful.  Laura Moen



More information about the Methods mailing list