TA cloning doesn't work if a certain base is at 5' end o

Robert Rumpf Rumpf.1 at osu.edu
Tue Sep 20 08:37:26 EST 1994


In article <aaa49db700015003391b@[140.254.12.85]>, rumpf.1 at osu.edu (Robert
Rumpf) wrote:

> Also of interest to anyone doing this type of cloning would be Gengxi Hu's
> article in DNA and Cell Biology 12(8) 1993, which specifies which bases are
> preferentially added when the 3' base is any of the 4 nucleotides, and this
> data is provided for 8 commonly used nucleotides.
> 
Err, change that to the 8 commonly used polymerases...heh heh...



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