TA cloning doesn't work if a certain base is at 5' end o

Robert Rumpf rumpf.1 at osu.edu
Tue Sep 20 08:10:57 EST 1994

Also of interest to anyone doing this type of cloning would be Gengxi Hu's
article in DNA and Cell Biology 12(8) 1993, which specifies which bases are
preferentially added when the 3' base is any of the 4 nucleotides, and this
data is provided for 8 commonly used nucleotides.

       Robert Rumpf          |"Do I have to tell a story/of a thousand
      rumpf.1 at osu.edu        | rainy days since we first met/it's a big
 The Ohio State University   | enough umbrella/but it's always me that
 Dept. of Molecular Genetics | ends up getting wet" -- Sting

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