Help- with lge scale phage prep

Mandalaparthy padmmv00 at ac.usfca.edu
Wed Sep 21 01:21:28 EST 1994


I am having problems making a good,clean  phage Dna prep. With liquid
lysis technique my Dna gets degraded very often. I am following the
protocol from Maniatis. Any help with ideas or protocols of LGE SCALE prep
reliable anquick would be appreciated. If any small changes in the
Maniatis Protocol work , that would also help a great deal. I have just
started work with the UN-DEPENDABLE phages!!

Please post or e-mail to padmmv00.dons.ac at usfca.edu

Padmaja Mandalaparthy

Grad student
USF-CA.



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