help with 5' race
ax041 at FreeNet.Carleton.CA
Wed Sep 21 11:48:53 EST 1994
We have tried twice now to get our 5'end for our sequence using
race protocole. I just saw in the Gibco catalog that there is an
RNAase H step to degrade the RNA after the reverse transcription.
Is this absolutely necessary?
The two times we tried the procedure all the clones were non
specific. We are getting very frustrated. Any advice will be
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