RNA tips (DEPC and nucleic acid)

Sean Eddy sre at al.cam.ac.uk
Wed Sep 21 16:03:07 EST 1994

In article <anderson-190994103333 at> anderson at pharmdec.wustl.edu (Eric C. Anderson) writes:
  >which is why when i make up DEPC solutions, i autoclave them first, _then_
  >add the DEPC once they cool down.

I think that's a risky way to go. DEPC in your buffers is bad news.
Trashes proteins and trashes RNA. DEPC-treat first, autoclave after.
Better yet, don't DEPC treat at all - just be paranoid about your
water source and your reagents.

- Sean Eddy
- MRC Laboratory of Molecular Biology, Cambridge, England
- sre at mrc-lmb.cam.ac.uk

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