Trizol reagent problems
devitta at uk.ac.birmingham
Thu Sep 22 10:40:51 EST 1994
In article <dpetersn.100.04C88780 at unixg.ubc.ca>, dpetersn at unixg.ubc.ca
(Murali Srinivasan) wrote:
> I've been trying to isolate total RNA from gram negative bacterial cells
> the TriZol reagent. My yields are extremely low. I'd like to increase the
> cell concentration, but protocol says use 1 ml per 1x107 cells. Perhaps the
> cells just aren't lysing and I should grind in liquid-nitrogen first??
> Anyone else have similar problems? Suggestions?
this can be a common problem. I recommend the acid phenol method for
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