Trizol reagent problems

Chutney devitta at uk.ac.birmingham
Thu Sep 22 10:40:51 EST 1994


In article <dpetersn.100.04C88780 at unixg.ubc.ca>, dpetersn at unixg.ubc.ca
(Murali Srinivasan) wrote:

> I've been trying to isolate total RNA from gram negative bacterial cells
using 
> the TriZol reagent.  My yields are extremely low.  I'd like to increase the 
> cell concentration, but protocol says use 1 ml per 1x107 cells.  Perhaps the 
> cells just aren't lysing and I should grind in liquid-nitrogen first??
> Anyone else have similar problems?  Suggestions?
> 
> Daniel

this can be a common problem.  I recommend the acid phenol method for
overall speed.



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