PCR Clean up, 150bp frag???
pumiglk at aa.wl.com
Thu Sep 22 19:16:02 EST 1994
In article <CwHn85.CLE at news.unr.edu>, jb at scs.unr.edu (Jon Burrows) wrote:
> Can anyone advise me on the most efficient way to clean up a 150bp PCR
> product from multiple rxns..I'm trying to get 50micrograms. Typical resin
> systems don't work well <3% recovery and I'm loathe to cut bands or DEAE
> from a prep gel, although this may be the way to go.
> Thanks Jon.
> + Jon Burrows. jb at shadow.unr.edu +
> + University of Nevada, Reno. +
I had reasonable recovery (80%) with a product called "Mermaid". Sort of a
redesign of Geneclean for small fragments. It is available from Bio101 (No
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