Linear polyacrylamide as an alternative to glycogen carrier

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Fri Sep 23 09:42:31 EST 1994


Duncan at genesys.demon.co.uk (Duncan Clark) writes:

>My only query is how toxic is acrylamide when polymerised (without bis 
>acrylamide). Linear polyacrylamide is made by polymerising a 5% acrylamide 
>solution in 40mM Tris, 20mM Na acetate, 1mM EDTA pH7.8 together with 
>1/100 vol of 10% ammonium persulphate and 1/1000 vol. TEMED. When the 
>solution becomes viscous (about 15-30mins) ppt polymer with 2.5volumes 
>EtOH, centrifuge and redissolve pellet in 20 vol. water O/N. Use 10ul 
>(25ug) per EtOH pptation. It works great and is inert, unlike glycogen, 
>but is it nasty? Anyone know?

My guess is that since it's polymerised it's OK. It's the UNpolymerised 
stuff that we have to watch out for.

-- 
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               



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