cDNA Challenge*************

[Shahram Mori]

Dear Molbionetters,
I have been using the Perkin Elmer RNA PCR kit to make cDNA from my Total
RNA samples. My RNA is in good condition and has been DNase treated. I
follow the protocol in GeneAMP kit and yet achieve no cDNA's. My
concentration of RNA is 200ng. As this is being done in a procaryotic
system, I am using random hexamers for cDNA synthesis. The time of
synthesis is 15 minutes at 42C. The dNTP concentration is 1mM. Anybody
that has any recomendations as to how I can improve this reaction , I
would be grateful to. 
Thanks a milion,
cheers
 
-- 
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||