Reverse Northern Blot?

Dr. YL Du yd128 at albnyvms.bitnet
Mon Sep 26 15:42:15 EST 1994


Hello:  I have a question for the Reverse Northern Blot.  Basically, I
want to use reverse transcriptase , poly T oligo, dNTP, and 32p-dCTP to
synthesize labeled cDNA.  Then using this as probe to hybridize with
spotted plasmids on nylon membrane.  Is there a protocol for this?  Any
experience?  All of suggestions are welcome.  Thanks.



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