Km of restriction enzymes

John H McDonald mcdonald at
Mon Sep 26 16:00:14 EST 1994

Does anyone know the Km for DNA of a "typical" restriction enzyme (or of
AccI in particular)?  I'd like to digest a rather small amount of DNA, and
I'm wondering if I need to add some other, generic DNA in order for the
enzyme to work.  I'm hoping to digest one of two alleles in a heterozygous
individual, then PCR amplify the uncut allele. 

John H. McDonald
Department of Biology
University of Delaware

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